A newly published study reports a first-ever open-field release of a self-limiting, genetically engineered diamondback moth, stating that it paves the way for an effective and sustainable approach to pest control.
The diamondback moth, also known as Plutella xylostella, is highly damaging to brassica crops such as cabbage, broccoli, cauliflower and canola. This new strain of diamondback moth, developed by Oxitec Ltd., is modified to control pest diamondback moth in a targeted manner. The study showed the engineered strain had similar field behaviors to unmodified diamondback moths, with results offering promise for future protection of farmers’ brassica crops.
The Cornell study was led by Professor Anthony Shelton in the Department of Entomology at Cornell University’s AgriTech in New York and has been published in Frontiers in Bioengineering and Biotechnology.
Oxitec’s self-limiting diamondback moth is modified to control its pest counterparts in the field. After release of males of this strain, they find and mate with pest females, but the self-limiting gene passed to offspring prevents female caterpillars from surviving. With sustained releases, the pest population is suppressed in a targeted way. After releases stop, the self-limiting insects decline and disappear from the environment within a few generations.
The field test builds on previously published work in greenhouses by Shelton and colleagues that demonstrated sustained releases of the self-limiting strain effectively suppressed the pest population and prevented resistance developing to an insecticide.
Male moths as a crop protection solution
Employing field and laboratory testing, as well as mathematical modelling, the researchers gathered relevant information on the genetically engineered strain of diamondback moth, whose wild counterparts cause billions of dollars in damage. The study was the first in the world to release self-limiting agricultural insects into an open field.
“For the field study, we used the ‘mark-release-recapture’ method, which has been used for decades to study insect movement in fields. Each strain was dusted with a fluorescent powder to mark each group before release, then captured in pheromone traps and identified by the powder colour and a molecular marker in the engineered strain,” explains Shelton.
“When released into a field, the self-limiting male insects behaved similarly to their non-modified counterparts in terms of factors that are relevant to their future application in crop protection, such as survival and distance travelled. In laboratory studies they competed equally well for female mates,” says Shelton. “Our mathematical models indicate that releasing the self-limiting strain would control a pest population without the use of supplementary insecticides, as was demonstrated in our greenhouse studies.”